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 Table of Contents  
ORIGINAL ARTICLE
Year : 2016  |  Volume : 3  |  Issue : 1  |  Page : 1-6

Role of histochemical stains in differentiating hemangioma and vascular malformation


Department of Pathology, Dr.D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, Maharashtra, India

Date of Web Publication14-Jun-2016

Correspondence Address:
Ruchir Jitendra Patel
36 Jalvihar Park, Malpur Road, Modasa - 383 315, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2349-6029.184009

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  Abstract 

Background: Benign vascular lesions such as vascular malformation and hemangioma at times pose difficulty in diagnosis both for clinicians and pathologists. Vascular malformations are difficult to treat while hemangiomas resolve spontaneously in most instances. There are instances when vascular malformations, especially arteriovenous malformations (AVMs) have been misdiagnosed as hemangiomas and vice-versa. Clinical and radiological correlation with histopathological confirmation of these anomalies is important for the management of these lesionsAim: The aim was to study the histological characteristics of hemangiomas and vascular malformations and to study the utility of histochemical stains in their diagnosis. Materials and Methods: We retrospectively studied fifty cases retrieved from the records of Department of Pathology which were diagnosed as hemangioma (n=32) and vascular malformation (n=18) on Hematoxylin and Eosin (H and E) stain over a period of 18 months. The cases were analyzed based on findings of histochemical stains such as Verhoeff-van Gieson (VVG), Masson's trichrome (MT), and toluidine blue. Results: After reviewing all the cases with the use of histochemical stains, two of the three cases originally diagnosed as hemangioma turned out to be AVM and one to be venous malformation. An increased number of intra-lesional nerves were found in 16 of 19 cases of AVM and in both cases of venous and lymphatic malformation. Hemangiomas did not show increase in nerve bundles. Mast cells were found to be increased in proliferating hemangiomas and pyogenic granulomas as compared to AVMs. Conclusion: Hemangiomas and vascular malformations should be clearly differentiated to reduce the risk of treatment failure and recurrence. With the use of histochemical stains such as VVG, MT and toluidine blue, the diagnostic difficulty can be reduced and definitive diagnosis is possible.

Keywords: Hemangioma, Masson's trichrome, toluidine blue, vascular malformation, Verhoeff–van Gieson


How to cite this article:
Patel RJ, Buch AC, Chandanwale SS, Kumar H. Role of histochemical stains in differentiating hemangioma and vascular malformation. Indian J Dermatopathol Diagn Dermatol 2016;3:1-6

How to cite this URL:
Patel RJ, Buch AC, Chandanwale SS, Kumar H. Role of histochemical stains in differentiating hemangioma and vascular malformation. Indian J Dermatopathol Diagn Dermatol [serial online] 2016 [cited 2019 Feb 17];3:1-6. Available from: http://www.ijdpdd.com/text.asp?2016/3/1/1/184009


  Introduction Top


Hemangiomas are very common tumors, characterized by increased numbers of normal or abnormal vessels filled with blood. They may be difficult to distinguish from vascular malformations.[1] Vascular malformations are abnormal clusters of blood vessels that occur during fetal development. The exact cause of these malformations remains unknown. Hemangioma and vascular malformation are both vascular anomalies with different outcomes. International Society for the Study of Vascular Anomalies has further classified vascular lesions into vascular neoplasm and vascular malformation. Vascular neoplasms include infantile hemangioma, congenital hemangioma, Kaposiform hemangioendothelioma, spindle cell hemangioendothelioma, epithelioid hemangioendothelioma, angiosarcoma and acquired benign vascular tumor like pyogenic granuloma. Vascular malformations are further classified into slow flow malformations, which include capillary, venous and lymphatic malformations and the fast flow ones which include arterial, arteriovenous, and combined malformations.[2] Infantile hemangiomas usually occur few weeks after birth, mainly on the head and neck region with female predominance (M:F = 1:3). Histologically they are characterized by rapid proliferation followed by involution.[3] Surgery may be required in up to 40% cases due to various reasons such as incomplete involution, cosmetic reasons, obstruction to vision, bleeding, infection, pain and rarely cardiovascular complications.[4] Vascular malformation is a rare vascular anomaly characterized by malformed vascular channels.[5] It occurs since birth, increases in size and grows proportionate to the body growth. They become symptomatic at any age due to complications and/or accelerated growth triggered by trauma and hormonal influence, which require surgical and medical intervention. Skin, brain, spinal cord and visceral organs are the common sites affected. Managing vascular malformation is a challenge to a surgeon. Incomplete resection or treatment failure can even lead to recurrence.[6] Thus, it is of utmost importance to diagnose vascular malformations and hemangiomas correctly and early in the course of the disease.

Histopathologically, hemangiomas show capillaries with endothelial proliferations, while vascular malformations show mature but dysplastic vessels which infiltrate into the deeper tissues. The histopathological diagnosis of malformation is supported by directly communicating arteries and veins, with presence of abnormal vessel wall with disrupted internal elastic lamina and eccentric intimal fibrous thickening in artery, arterialization of veins in arteriovenous malformation (AVM) and presence of varying size and thickness of abnormal venous channels with disrupted or abnormal vessel wall in venous malformation.[7] AVMs can be confirmed by demonstrating elastic tissue of arteries and arterioles using Verhoeff van-Gieson (VVG) and orcein stains. Recent studies have shown the presence of increased intralesional nerves in vascular malformations as an additional tool in diagnosing vascular malformation.[8] This prompted us to study and review the cases of vascular malformations and hemangiomas diagnosed in our department. The aim of this study was to differentiate hemangioma and vascular malformation based on histopathological features and to assess the utility of various histochemical stains such as VVG, Masson's trichrome (MT) and toluidine blue in differentiating these lesions.


  Materials and Methods Top


A total of fifty cases were retrieved from surgical pathology records. The lesions diagnosed as vascular malformation (n=18) and hemangioma (n=32) on hematoxylin and eosin (H and E) stained sections were included in the study. Vascular malformations included AVM,(n=17) and lymphatic malformation (n=1). Hemangiomas included infantile hemangiomas (n=4), capillary hemangiomas (n=14) and pyogenic granuloma (n=14). Vascular tumors diagnosed as hemangioendotheliomas and angiosarcomas were excluded from the study. Institute Ethics Committee clearance was taken for the study. Relevant clinical history including age, sex, and site of lesion were collected. However, follow-up was not available. One case of AVM had a history of recurrence. Formalin-fixed, paraffin-embedded tissue blocks were made and tissue sections were cut at 4µ for special stains. Routine H and E stain was performed and special histochemical stains VVG, MT and toluidine blue were done in all cases as per standard guidelines.[9] VVG stain was used for identification of arteries and arterioles and MT stain for identification of various soft tissue components such as nerves, smooth muscles and vessels. The presence of intra-lesional nerve bundles was scored as follows:-score 0 if there was no increase in nerves, score 1 if there was focal and slight increase in nerves and score 2 if there was multifocal or diffuse increase in nerve.[8] The mast cell density was counted in toluidine blue-stained sections using Olympus CX 21 model microscope in all the cases using oil immersion objective andx10 eyepiece. With these specifications, the magnification isx1000 and field view diameter is 0.20 mm. Therefore, area of the field view using the formula for the area of a circle = πr2 (where r is the radius of a circle) is 0.0314 mm2. Thus, for counting in 1 mm2 area, we have to do the cell count in 1/0.0314=31.84 fields, or rounded up to 32 fields. Therefore, 32 non-overlapping fields were counted to obtain mast cell count per mm2 area. In each representative section, 32 fields were counted to obtain the number of cells per mm2.


  Results Top


As per the surgical record diagnosis, there were 32 cases of hemangioma [Figure 1]a and 19 cases of vascular malformation (AVM n=17 [Figure 1]b, lymphatic malformation n=1) diagnosed on H and E. Of 32 cases diagnosed as hemangiomas, two cases were reclassified as AVM as they revealed arteries and/or arterioles and increased nerve bundles on VVG and MT stains [Figure 2]a [Figure 2]b,[Figure 2]c. Myelinated fibers appeared red with green endoneurial sheath [Figure 2]d. One case of hemangioma was also reclassified as venous malformation as it revealed malformed venous channels without arteries/arterioles. It also showed increase in small nerve bundles. Reclassified AVMs and venous malformation also revealed increase in nerve fibers. All other originally diagnosed cases of vascular malformation were also reconfirmed on VVG and MT. After this reclassification, there were a total 29 cases of hemangiomas, 19 of AVM and one case each of venous and lymphatic malformation. Sex ratio in hemangioma and vascular malformation were 1.00:1.75(M:F) and 1:1(M:F), respectively. Hemangiomas were seen in all age groups ranging from 12 months to 60 years (mean = 25 years). The age in vascular malformation ranged from 9 months to 75 years (mean age = 21 years). Increased intra-lesional nerves/bundles were present in 18 of 21(85.66%) cases of vascular malformation (16/19 AVM, 1/1 lymphatic malformation, and 1/1 venous malformation), whereas these were not increased in any case of hemangioma. The increase in nerves was further scored as shown in [Table 1]. The salient histochemical findings to differentiate hemangioma from vascular malformation are highlighted in [Table 2]. Mean mast cell density calculated with toluidine blue stained sections was 42.23/mm2 and 29.56/mm2 in hemangiomas and vascular malformations, respectively [Figure 3]a and [Figure 3]b.
Figure 1:(a) Capillary hemangioma containing congested and dilated capillaries of varying size (H and E, ×100),(b) arteriovenous malformation showing artery (thick arrow), vein (arrowhead) and a nerve bundle (thin arrow) (H and E, ×100)

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Figure 2: Capillary hemangioma reclassified as arteriovenous malformation based on special stains (a) artery (thick arrow) adjacent to small capillary-like proliferations (thin arrow)(H and E, ×100),(b) Verhoeff–van Gieson stain highlighting internal elastic lamina of artery (arrow) (Verhoeff–van Gieson, ×100), (c) increased nerve bundles (arrowhead) and tortuous artery (thick arrow), dysplastic vein (thin arrow)(Masson's trichrome, ×100), (d) nerve bundle containing multiple myelinated axons (red) separated by endoneurial sheath (green)(Masson's trichrome, ×400)

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Table 1: Vascular malformation site and scoring of nerve fibers/bundles

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Table 2: Salient histochemical features of hemangioma and vascular malformation

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Figure 3:(a) Increased number of mast cells (purple) in hemangioma (b) relatively less number of mast cells in arteriovenous malformation (toluidine blue, ×400)

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  Discussion Top


Hemangiomas are benign vascular tumors, characterized by endothelial hyperplasia and rapid growth. They respond to corticosteroids. Vascular malformations are characterized by vascular dysmorphogenesis and normal endothelial turnover. They never regress and require medical and surgical treatment. If vascular malformation is misdiagnosed as hemangioma, it will lead to treatment failure and a possible recurrence. Therefore, correct diagnosis of both these lesions is essential.[6]

We found that hemangiomas were more common in females than males; however, there was no sex predilection in vascular malformations. The sites of vascular malformations and hemangiomas in our study were similar to those described in literature, although age varied significantly in hemangiomas as compared to vascular malformations. This variation could be explained by the inclusion of cases of lobular capillary hemangiomas (pyogenic granuloma) which are acquired lesions occurring in adults.[10]

Various studies in the past have evaluated the use of elastic stains for identification of arteries/arterioles. Koutlas and Jessurun used orcein stain to study the presence and distribution of elastic fibers in the vascular components of arteriovenous hemangiomas.[11] Adegboyega and Qui used Movat pentachrome stain to identify various tissue components in hemangiomas and AVMs.[12] Pawane et al. used VVG stain in 44 cases of AVMs.[13] In our study, VVG and MT helped to re-classify two cases of hemangioma as AVM and one case of hemangioma as venous malformation.

Routine H and E stain can identify nerves as wavy bundles. We used MT stain to identify and evaluate nerves. We found nerve bundles in 85.66% (18 out of 21) cases of vascular malformation (16/19 AVMs, 1/1 lymphatic malformation and 1/1 venous malformation). Adegboyega and Qui used immunohistochemical stain S-100 to assess the presence of nerves and nerve fibers in hemangiomas and vascular malformations. Intra-lesional nerves were identified in 91% (69 out of 76) cases of AVMs.[12] In contrast, nerve bundles were not found to be increased in any of the 91 cases of hemangioma. Pawane et al. identified intra-lesional nerves with H and E in 81.8%(36 cases) of AVM, whereas they were seen in only 6% cases (three cases) of hemangioma.[13] Our findings are in agreement with Adegboyega and Qui's study and Pawane et al. and various other studies.[12] It has been proposed that peripheral blood vessels and nerves share patterning mechanism during development. This could explain increase in nerve bundles in vascular malformations.[14]

We also support Meijer-Jorna et al.[8] observation that AVMs on head and neck and upper extremities show relatively more nerves than trunk and lower extremities [Table1]. It is postulated that the congruency of the nervous system and the vascular system differs according to the location as seen in experimental studies.[15]

We found that on H and E stain, it may be difficult to differentiate between small arterioles and large capillaries with fibro-lamellar hyperplasia. Even elastic stain may not be helpful to differentiate between them as internal elastic lamina may be inconspicuous or absent in small arterioles. Presence of continuous wreath of concentric smooth muscle layer in arteries and arterioles as compared to discontinuous wreath of smooth muscle bundles in veins can be used as a feature to differentiate between them. This can be done easily by MT and/or VVG [Figure 4]a].[16] We could easily identify dysplastic vessels and study their characteristic features such as leaky vessels, eccentric intimal fibrosis of artery, arterialization of vein, and disrupted internal elastic lamina in these vascular malformations [Figures4 and 5a]. Thus, we found that histochemical stains play an important role in identifying different type of vessels with characteristic histological features.
Figure 4: Arteriovenous malformation (a) arteriole with concentric smooth muscles (Masson's trichrome × 400),(b) dysplastic vein (thin arrow) with varying thickness and red blood cell extravasation (thick arrow)(Verhoeff–van Gieson, ×100),(c) eccentric intimal fibrosis of artery (Masson's trichrome, ×100) and (d) luminal occlusion of arterialized vein (arrow)(Masson's trichrome, ×100)

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Various authors have utilized toluidine blue stain to study mast cells in hemangiomas and AVM and found that mast cell numbers are increased in hemangiomas and AVMs.[13],[17] We found mean mast cell density of 42.23/mm2 in hemangiomas and 29.56/mm2 in vascular malformations which ranged from 6 to 90/mm2 in hemangiomas and 4–60/mm2 in vascular malformations. Mast cells were found concentrated more near vessels than normal surrounding area. Various studies have shown that mast cells are decreased in involuting hemangiomas as compared to proliferating hemangiomas which suggest the role of mast cells in maintaining hemangiomas in proliferating stage.[13] In our study, there were no involuting hemangiomas. Although mast cells have a role in the pathogenesis of hemangiomas, mast cell density has not been found to be a useful marker to differentiate hemangiomas from AVM.[13] We also found increased mast cell density in both hemangiomas and vascular malformations (hemangioma>vascular malformations), but this increase was not statistically significant.

Non-involuting congenital hemangioma (NICH) is a rare, congenital cutaneous vascular anomaly which is fully developed at birth and histologically characterized by small, thin-walled vessels having hobnailing of the endothelial cells with a large and often stellate central vessel. They also contain interlobular veins and arteries which may be dysplastic. Differentiating NICH from AVM/venous malformation would certainly be difficult as dysplastic arteries and veins are seen even in NICH,[18] although, we did not have any case of NICH in this study. However we believe that presence of increased nerve bundles may help in differentiating even NICH from AVM on histological ground. It has been found that NICH is GLUT-1 negative and may show increase in mast cell density.[18] In addition, absence of direct venous filling in NICH on radiological investigation (ultrasonography[USG]) would help differentiating it from AVM/vascular malformation.[18] Thus, clinicopathological and radiological correlation is a must in such cases for definitive diagnosis.

We reclassified two cases of hemangioma in to AVM, based on large number of nerve bundles and vascular dysmorphogenesis observed on MT and VVG. The third case diagnosed as hemangioma was reclassified as venous malformation as MT stain revealed the presence of dysplastic venous channels with discontinuous smooth muscles in their walls in addition to the presence of capillary proliferations and increased nerves [Figure 5]b]. Endothelial and capillary-like proliferations may also be seen in vascular malformations as seen in this case. These capillary-like endothelial proliferations in vascular malformations are due to ischemia and vascular remodeling.[19],[20] GLUT-1 is a new immunohistochemical marker which has been recently utilized by various authors in differentiating hemangiomas and AVMs as it is found mainly positive in endothelial proliferation of infantile hemangiomas.[19],[21] Expression of Wilms tumor-1 gene distinguishes vascular malformation from proliferating endothelial lesions,[22] but availability and cost are limiting its utility in routine practice. Mast cells have a role in endothelial proliferation.
Figure 5:(a) Disrupted internal elastic lamina (black) of artery in arteriovenous malformation (Verhoeff–van Gieson, ×400),(b) hemangioma reclassified as venous malformation. Note dysplastic vein (thick arrow) with discontinuous and deficient smooth muscles in its wall and adjacent capillary-like endothelial proliferations and small nerve bundle (thin arrow)(Masson's trichrome, ×400)

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Although various authors have used these histochemical stains, these characteristic features of dysplastic vessels have not been highlighted as demonstrated in our study. We could even identify small nerve bundles with MT stain, which were easily missed on routine H and E stain. This definitely increased the sensitivity of nerve detection and helped further in nerve bundle scoring.

Radiological investigations, especially, USG plays an important role in classifying these lesions based on the flow of the lesion. Although clinical diagnosis and radiological correlation are sufficient for diagnosis in most of the cases, confirmation of all resected specimens is must because many times vascular malformations can simulate hemangiomas clinically and radiologically.[23] A few cases in our study were clinically misdiagnosed a lipoma or cyst. Few radiologically diagnosed cases of hemangioma were confirmed as AVM on histopathology. Hence, multidisciplinary approach and careful evaluation of each case of vascular lesion is essential for proper management.

Limitations

This was a retrospective study. In few cases, clinical and radiological correlation was not possible as they were not subjected to radiological investigation. These cases were diagnosed solely based on HPE. Although MT stain was used for identification of nerve bundles, S-100 immunostaining was not used for confirmation.


  Conclusion Top


Hemangioma and vascular malformation, especially AVM, should be clearly differentiated to reduce the risk of treatment failure and recurrence. With the use of histochemical stains such as VVG and MT, the diagnostic difficulty can be reduced and certain characteristic features can be easily studied and evaluated.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

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Mitchell RN, Schoen FJ. Blood vessels. In: Kumar V, Abbas AK, Fausto N, et al., editors. Robbins and Cotran Pathologic basis of disease. 8thed. Philadelphia, PA: Elsevier; 2009. p.520.  Back to cited text no. 1
    
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12.
Adegboyega PA, Qiu S. Hemangioma versus vascular malformation: Presence of nerve bundle is a diagnostic clue for vascular malformation. Arch Pathol Lab Med 2005; 129:772-5.  Back to cited text no. 12
    
13.
Pawane P, Anshu, Gangane N. Hemangiomas versus arterio-venous malformations: Role of elastic stains and mast cell density. Indian J Pathol Microbiol 2014; 57:191-5.  Back to cited text no. 13
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Mukouyama YS, Gerber HP, Ferrara N, Gu C, Anderson DJ. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 2005; 132:941-52.  Back to cited text no. 15
    
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Dalton SR, Fillman EP, Ferringer T, Tyler W, Elston DM. Smooth muscle pattern is more reliable than the presence or absence of an internal elastic lamina in distinguishing an artery from a vein. J Cutan Pathol 2006; 33:216-9.  Back to cited text no. 16
    
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Enjolras O, Mulliken JB, Boon LM, Wassef M, Kozakewich HP, Burrows PE. Noninvoluting congenital hemangioma: A rare cutaneous vascular anomaly. Plast Reconstr Surg 2001; 107:1647-54.  Back to cited text no. 18
    
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Mo JQ, Dimashkieh HH, Bove KE. GLUT1 endothelial reactivity distinguishes hepatic infantile hemangioma from congenital hepatic vascular malformation with associated capillary proliferation. Hum Pathol 2004; 35:200-9.  Back to cited text no. 19
    
20.
Hashimoto T, Mesa-Tejada R, Quick CM, Bollen AW, Joshi S, Pile-Spellman J, et al. Evidence of increased endothelial cell turnover in brain arteriovenous malformations. Neurosurgery 2001; 49:124-31.  Back to cited text no. 20
    
21.
Meijer-Jorna LB, van der Loos CM, de Boer OJ, van der Horst CM, van der Wal AC. Microvascular proliferation in congenital vascular malformations of skin and soft tissue. J Clin Pathol 2007; 60:798-803.  Back to cited text no. 21
    
22.
Lawley LP, Cerimele F, Weiss SW, North P, Cohen C, Kozakewich HP, et al. Expression of Wilms tumor 1 gene distinguishes vascular malformations from proliferative endothelial lesions. Arch Dermatol 2005; 141:1297-300.  Back to cited text no. 22
    
23.
Jakubowski LA, Chun RH, Drolet BA, Jensen JN, North PE. Misdiagnosed as infantile hemangioma: Early presentation of small vessel-rich A VM. Int J Pediatr Otorhinolaryngol Extra 2013; 8:71-4.  Back to cited text no. 23
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
 
 
    Tables

  [Table 1], [Table 2]



 

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