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  Indian J Med Microbiol
 

Figure 1: Active vitiligo. (a) Complete absence of melanin in basal cell layer with mild spongiosis (H and E, ×400). (b) The absence of melanin pigmentation on Masson Fontana (MF, ×400). Inset shows normal melanin pigment in the positive control section (MF, ×400). (c) human melanoma black-45 immunostaining showing no melanocytes in the basal layer (IHC, ×400). (d) Epidermis showing increased number of Langerhans cells on S-100 immunostaining (IHC, ×400)

Figure 1: Active vitiligo. (a) Complete absence of melanin in basal cell layer with mild spongiosis (H and E, ×400). (b) The absence of melanin pigmentation on Masson Fontana (MF, ×400). Inset shows normal melanin pigment in the positive control section (MF, ×400). (c) human melanoma black-45 immunostaining showing no melanocytes in the basal layer (IHC, ×400). (d) Epidermis showing increased number of Langerhans cells on S-100 immunostaining (IHC, ×400)