Indian Journal of Dermatopathology and Diagnostic Dermatology

REVIEW ARTICLE
Year
: 2016  |  Volume : 3  |  Issue : 2  |  Page : 29--41

Panniculitis: A dermatopathologist's perspective and approach to diagnosis


Parikshaa Gupta1, Uma Nahar Saikia1, Sandeep Arora1, Dipankar De2, Bishan Das Radotra2,  
1 Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Dermatology, Leprology and Venereology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Uma Nahar Saikia
Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh
India

Abstract

Panniculitis is the inflammation of subcutaneous fat. It poses a diagnostic challenge, both for the clinician as well as the histopathologist owing to its diverse etiologies, overlapping morphological features, and dynamic nature of the lesions. The standard protocol for histopathologic diagnosis is to examine deep skin biopsies after staining with hematoxylin and eosin. Sometimes, however, special stains are also needed to rule out varied etiologies. A thorough histopathologic examination in conjunction with appropriate clinical details is the key to identify different subtypes of panniculitis. In this review, we discuss the classification of panniculitis with salient histopathologic features of the subtypes in each category. We also present a diagnostic algorithm for arriving at a definitive histopathologic diagnosis of different panniculitis subtypes.



How to cite this article:
Gupta P, Saikia UN, Arora S, De D, Radotra BD. Panniculitis: A dermatopathologist's perspective and approach to diagnosis.Indian J Dermatopathol Diagn Dermatol 2016;3:29-41


How to cite this URL:
Gupta P, Saikia UN, Arora S, De D, Radotra BD. Panniculitis: A dermatopathologist's perspective and approach to diagnosis. Indian J Dermatopathol Diagn Dermatol [serial online] 2016 [cited 2022 Jun 26 ];3:29-41
Available from: https://www.ijdpdd.com/text.asp?2016/3/2/29/195224


Full Text



 Introduction



Panniculitis is the inflammation of the subcutaneous fat. It is regarded as the “ugly duckling” in dermatopathology, as it often poses diagnostic difficulties, both clinically as well as histopathologically.[1],[2],[3] There is considerable overlap, not only clinically but also histologically, among various types of panniculitis. The major reasons for the diagnostic difficulties include inadequate biopsy specimen, inadequate clinical details, overlapping histopathological features, and evolving morphology of different types of panniculitides at different stages. In our experience, inadequate skin biopsy specimens are the most common reason for diagnostic struggle in panniculitis followed closely by their overlapping histopathology. In patients with suspected panniculitis, a deep biopsy to include the subcutaneous fat is fundamental.[4],[5],[6],[7] We believe that with satisfactory specimens, adequate clinical history, and comprehensive histopathologic examination, a specific diagnosis may be decreed in most cases of panniculitis. The “gold standard” practice is to examine deeper sections through the block after staining with hematoxylin and eosin (H and E). Sometimes, however, special stains such as elastin stains, polarization of sections, or cultures of the tissue for pathogenic bacteria or fungi are also required for formulating a specific diagnosis.

There are three major components of the subcutis, i.e., lipocytes, blood vessels, and fibrous septa. The fat cells or lipocytes are large and appear as empty cells with signet-ring morphology on H and E stain. This is due to the displacement of the nucleus by a single, large intracytoplasmic, fat containing vacuole. The thin fibrous septa that divide the subcutaneous fat into lobules are constituted by collagen and reticulin fibers and contain the nerves, blood, and lymphatic channels. Each fat lobule is supplied by an arteriole that branches into capillaries in the lobule. There is a peculiar pattern of blood supply of the lobules. The blood supply is terminal so that there are no capillary communications between adjacent lobules or between dermis and subcutaneous fat. This explains why large-vessel vasculitis involving the septal vessels is commonly attended by little inflammation of the fat lobules, whereas small-vessel vasculitis displays extensive fat necrosis with centrilobular infarction and dense inflammation within the lobule.[8]

The subcutaneous fat has a poor repertoire of responses to noxious stimuli, with the most common response being fat necrosis on histopathology, which could in turn be secondary to innumerable conditions.[9],[10] It is also important to remember that the subcutaneous fat may be involved secondarily in vasculitides, trauma, deep cutaneous fungal infections, and many malignancies.[11],[12],[13],[14],[15],[16],[17],[18],[19],[20] Histopathologically, panniculitis can predominantly affect lobules or septa, with/without vascular involvement. Accordingly, the panniculitis can be classified into following main classes:

Predominantly septal panniculitis without vasculitis Predominantly septal panniculitis with vasculitis Predominantly lobular panniculitis without vasculitis Predominantly lobular panniculitis with vasculitis Others.

The detailed histopathologic classification of panniculitis is given in [Table 1].[3],[7],[8],[9]{Table 1}

 Septal Panniculitis Without Vasculitis



Erythema nodosum

Erythema nodosum (EN) is the prototype of nodular septal panniculitis. It is a clinical syndrome with varied symptoms and etiologies. It typically affects young adults with a predilection for females. There are two clinical forms of EN - acute and chronic, which differ in their clinical manifestations but do not exhibit much histopathological difference. Acute EN also known as migratory panniculitis or subacute nodular migratory panniculitis is typically asymmetrical, unilateral, and predominantly involves legs. It exhibits a female preponderance and tends to affect older age groups, in contrast to classical EN. The chronic form is less recognized, however, shows similar nodular lesions developing over months or even years [Figure 1].[21],[22],[23],[24]{Figure 1}

Histologically, EN shows widening of the septa due to edema and neutrophilic infiltrate admixed with eosinophils. The infiltrate often extends into the periphery of the fat lobules and between the individual fat cells in a lace-like fashion in early stages [Figure 2]a. Vascular changes and hemorrhage are commonly seen but no definite vasculitis is present. In later stages, the infiltrate is replaced by collections of histiocytes, especially around slit-like spaces or blood vessels forming Miescher's radial nodules, which are considered to be a characteristic feature of EN and can be found in all stages of EN. Further progression of the lesion leads to the development of a granulomatous response with giant cells [Figure 3]. However, fat necrosis is usually not seen. The older lesions of EN show widening of the septa due to fibrosis and inflammation at the edges of the septa and mainly involve the periphery of the fat lobules. The inflammation is rich in macrophages, and neutrophils are usually absent. Macrophages at the edges of the fat lobules show phagocytosis of lipid from the damaged adipocytes. Occasionally, loosely formed residual granulomas can be seen in the septa along with the multinucleated giant cells [Figure 3]. The vascular changes are less prominent. The oldest lesions have septal widening and fibrosis with minimal inflammatory cell infiltrate [Figure 2]b. EN migrans characteristically shows densely scarred and thickened interlobular septa with conspicuous granulomas and giant cells.[7],[25],[26]{Figure 2}{Figure 3}

The differential diagnosis of EN includes erythema induratum of Bazin (EIB), superficial migratory thrombophlebitis, nodular vasculitis (NV), subcutaneous tuberculosis, necrobiosis lipoidica (NL), deep morphea, subcutaneous granuloma annulare (SGA), rheumatoid nodule, and necrobiotic xanthogranuloma.[27],[28],[29],[30],[31],[32],[33],[34] Although EIB and NV predominantly exhibit lobular panniculitis, however, features of septal panniculitis can also be observed, and hence they often sham striking morphologic resemblance to EN. Vasculitis and zones of fat necrosis are absent in EN and frequent in EIB.[27] NV has mainly lymphocytic infiltration with fibrous thickening and obliteration of vascular lumina.[28],[29],[30] In contrast to EN, superficial migratory thrombophlebitis has a large vein with luminal thrombus. In subcutaneous tuberculosis, the upper portion of the panniculus is spared, whereas it is involved in EN.[31],[32] Furthermore, in subcutaneous tuberculosis, stains for acid-fast organisms and cultures are useful.[33],[34]

As the histopathologic findings of EN can be quite variable, clinical correlation is of paramount importance. However, all biopsies should be accompanied by cultures and special stains for bacteria, fungi, and atypical mycobacteria. In addition, presence of foreign material should also be looked for to rule out other causes of EN.[25],[26],[27],[28],[29],[30],[31],[32],[33],[34]

Necrobiosis lipoidica

NL is an idiopathic rare skin disorder mainly affecting the skin of insulin-dependent diabetic females; however, nondiabetic individuals may also be affected. The lesions are usually bilateral, the most common site being lower extremities mainly calves, ankles, thighs, popliteal region, and feet [Figure 4]a.{Figure 4}

The histopathology is characteristic with variable degree of granulomatous inflammation around degenerated collagen in the dermis with the involvement of subcutaneous fat. The degenerated collagen appears paler with gray hue and more haphazardly arranged on H- and E-stained sections. This alteration in the collagen is known as necrobiosis. Occasionally, there may be few scattered multinucleated or Langhans giant cells and histiocytes with extensive sclerosis. The epidermis is usually normal, may be atrophic or hyperkeratotic [Figure 5].[35],[36] Sometimes, it can be difficult to distinguish NL from granuloma annulare (GA) and rheumatoid nodules though there is no increased mucin in NL and plasma cell collections can be seen in the deeper dermis.[7],[25],[26]{Figure 5}

Scleroderma

The word scleroderma is derived from skleros - hard and derma - skin (Greek) is a connective tissue disorder, which leads to thickening and fibrosis of the skin. There are two types of scleroderma, i.e., localized (morphea) and systemic (progressive systemic sclerosis). The thickening and hardening of skin may also be associated with metabolic, genetic, immunologic, or occupational disorders.[37]

In nearly all forms of scleroderma, the inflammatory changes involve both the dermis as well as the subcutaneous fat and cannot be differentiated histologically. In the localized form, early stages show lymphoplasmacytic perivascular infiltrate throughout the dermis, extending into thickened fibrous septa along with hyalinosis and thickened collagen bundles. In the later stages, the inflammatory infiltrate extends from the septa into the periphery of the fat lobules causing reduction in the fat lobules, which is replaced by newly formed pale collagen. In the late sclerotic stage, the inflammation disappears and the collagen bundles in the reticular dermis are thickened, hypocellular and stain more deeply eosinophilic. The eccrine glands too appear atrophic and surrounded by newly formed collagen [Figure 6].[7],[26]{Figure 6}

Subcutaneous granuloma annulare

SGA is an idiopathic condition characterized by palisaded granulomatous infiltration in the dermis commonly occurring in children and young adults with female predilection. The common sites of involvement are hands, arms, legs, and feet. It is a chronic condition with variants including (a) generalized popular form, (b) perforating, (c) erythematous, and (d) deep GA which involves the subcutis.[38],[39]

Histologically, GA shows infiltration of histiocytes palisading the areas of mucin [Figure 7]a. Increased mucin is a hallmark feature of GA and is visible as faint blue finely granular material on H and E stain. Special stains including Alcian blue and colloidal iron are essential to demonstrate increased mucin [Figure 7]b. In addition, there is perivascular lymphocytic infiltrate seen in the dermis and within the septa. The differential diagnosis includes NL where there is no or less mucin and rheumatoid nodules, which contain more of fibrin.[7],[25],[26]{Figure 7}

Rheumatoid nodule

Rheumatoid nodules occur in about 20%–25% patients with rheumatoid arthritis with high rheumatoid factor titer. They are usually situated near bony structures close to a joint over extensor prominences. The lesions are deep-seated masses in the subcutaneous tissue and vary in size from few mm to 5 cm.[40],[41],[42]

Histologically, there is fibrinoid degeneration of collagen in the deep dermis and subcutis surrounded by a palisading of histiocytes [Figure 8]. This degenerated collagen stains deep red on H and E stain accompanied with nuclear fragments and basophilic material. The mucin is always minimal or absent. The stroma surrounding the nodules shows foreign body giant cells and perivascular lymphocytic infiltrate including plasma cells. Acute or chronic thrombotic endarteritis is observed in some cases along with vascular proliferation. Old lesions show dense fibrosis, clefts, and cystic degeneration.[7],[8]{Figure 8}

Accelerated rheumatoid nodulosis is an entity characterized by rapid development of multiple periarticular subcutaneous nodules after institution of methotrexate therapy for rheumatoid arthritis. Regression of nodules is noted after stopping methotrexate. Pseudorheumatoid nodule mimics RN and the term is often used interchangeably with SGA. It is seen commonly in children and patients without a history of joint pains, RA, or systemic lupus erythematosus (SLE).[43]

 Septal Panniculitis With Vasculitis



Cutaneous polyarteritis nodosa

Cutaneous polyarteritis nodosa is a variant of polyarteritis nodosa (PAN), which classically involves deep dermis and subcutaneous fat with a classical feature of arteritis. It is more common in men and presents with skin nodules, which are associated with livedo reticularis, fever, and arthralgia. Renal involvement occurs in 75% of cases and is the most common cause of mortality. However, the disease can be limited to skin with a long clinical course of multiple recurrences and have a good prognosis.[44],[45],[46],[47],[48]

Microscopically, the characteristic lesions show panarteritis involving the medium- to small-sized muscular arteries. There is necrotizing leukocytoclastic vasculitis with fibrin deposition and partial to complete destruction of the elastic lamina [Figure 9]. The infiltrate is predominantly neutrophilic with admixed eosinophils in and around the vessels involving the panniculus causing lobular panniculitis. In the later stages, there is intimal proliferation with thrombosis leading to ischemia and ulceration sometimes. In the healed or healing stage, there is fibrovascular proliferation and nonspecific lymphocytic infiltrate in the perivascular area.[7],[8] In a series of 16 cases with cutaneous PAN, it was observed that all cases manifested lesions in the form of nodules and/or indurated erythematous patches over their lower extremities, and on histopathologic examination, all cases exhibited necrotizing vasculitis of small, subcutaneous, muscular arteries with/without luminal occlusion. Eight of these cases were followed up for at least 5 years, with the longest follow-up period being 19 years and displayed good prognoses without any systemic involvement.[46]{Figure 9}

Superficial thrombophlebitis

Superficial thrombophlebitis usually involves lower extremities, presenting as tender, erythematous nodule, or cord-like structure. Often there is the presence of predisposing factors such as varicose veins, hypercoagulable state, oral contraceptive intake, or underlying malignancy.[49],[50],[51]

There is involvement of small- or medium-sized veins in the deeper dermis and subcutaneous fat causing acute vasculitis and thrombosis occluding the lumina.[52],[53],[54],[55] In early stages, there is leukocytic infiltrate accompanied with edema, and in later stages, lymphocytes, histiocytes, and occasional giant cells are seen in the wall of the veins.[7],[29] The differential diagnosis includes PAN; however, there is no involvement of arteries. A Gram stain is always advised to rule out common bacteria causing suppurative thrombophlebitis, and a history of trauma is needed to exclude traumatic fat necrosis.[52],[53],[54]

 Lobular Panniculitis Without Vasculitis



Lupus erythematosus panniculitis (lupus profundus)

This is defined as a specific involvement of the subcutaneous fat in patients with lupus erythematosus (LE). It is a rare manifestation of the disease, occurring approximately in 1%–3% of patients with cutaneous LE. It may be observed in patients with discoid LE or SLE or as an isolated phenomenon without systemic or other cutaneous findings [Figure 1]a. A patchy lymphoplasmacytic infiltrate in the lobules of the subcutaneous fat along with lymphocytic nuclear dust is a clue for the histopathological diagnosis of LE panniculitis (LEP) [Figure 10]. The diagnosis of LEP may be especially problematic in cases where the involvement of subcutaneous fat is the only manifestation of the disease. Clinically, LEP and subcutaneous panniculitis-like T-cell lymphoma (SPTCL) are indistinguishable; however, in SPTCL, the presence of atypical CD8-positive T-lymphocytes and the absence of septal fibrosis, B-cell follicles, and plasma cells clinch the diagnosis.[53],[54],[55],[56],[57],[58]{Figure 10}

Sclerosing panniculitis

Sclerosing panniculitis also known as lipodermatosclerosis often affects middle-aged or elderly, overweight females with accompanying history of varicose veins, thrombophlebitis, and deep vein thrombosis. The main pathogenic mechanism is venous stasis, and hence the condition is also called stasis-associated sclerosing panniculitis.

The histopathological features are variable depending on the stage of the disease. Early lesions show centrilobular ischemia and infarction of fat cells in the subcutaneous fat along with vascular congestion and hemorrhage. No vasculitis is seen though thrombosis can be present. Lymphocytic infiltrate in the septa with hemosiderin-laden macrophages is commonly present. In later stages, there is hyalinization of the fat lobules with microcystic change and fat necrosis. Advanced lesion would reveal fibrous scarring of the septa with atrophy of the fat lobules. There are features of vessel wall thickening, chronic inflammation, and lobular capillary proliferation and fibrosis in the dermis. The differential diagnosis includes morphea profunda and scleroderma in the late stages.[7],[59],[60],[61]

Traumatic panniculitis

The trauma may be physical including blunt trauma, cold, excessive heat or electrical injury, or chemical injury produced by injection of noxious substances. There is quite a wide histological variation encountered in panniculitis due to physical effects (e.g., cold and pressure) or trauma to the skin.[62],[63],[64]

Factitial panniculitides

Factitial panniculitides are by no means rare and should be considered in any panniculitis where there are unusual features.[65] The chemical injury causes acute inflammation with focal fat necrosis and hemorrhage. Older lesions will show lymphocytic infiltrate accompanied with macrophages and fibrosis. There is no vasculitis or thrombosis. Injection of oily compounds leads to accumulation of foamy macrophages with formation of fatty cysts with surrounding fibrosis giving “Swiss Cheese appearance.” Similar appearance is seen in sclerosing lipogranuloma. The subcutaneous tissue of children's is more sensitive to cold injury, which can cause nodules of cold injury on applying ice cubes to the skin.[7],[62]

Subcutaneous fat necrosis of the newborn

Subcutaneous fat necrosis of newborn occurs as a complication of forceps delivery or cesarean section in neonates. It presents as erythematous to violaceous plaques within weeks of the birth. The lesions usually subside after few weeks, however, may form nodules.

Microscopically, they show focal fat necrosis in the lobules with macrophage infiltration and foreign body type of giant cells. The macrophages and giant cells contain crystalline fat, which appear as needle-shaped clefts arranged radially. Focal small calcium deposits may also be seen.[7],[66],[67],[68],[69],[70] An important morphological differential to be remembered here is the Gouty panniculitis, wherein the needle-shaped monosodium urate monohydrate crystals are seen in the soft tissues surrounded by foreign body giant cell reaction.

Sclerema neonatorum

This is a very rare disorder commonly seen in premature infants. The characteristic appearance shows diffuse, rapidly spreading, nonpitting hardening of the subcutaneous adipose tissue of the neonates in the first few days of life accompanied with cyanosis. There is waxy appearance of the skin that is cold and tight. The condition is lethal if left untreated. The subcutaneous tissue is thickened due to increase in fat cells and wide fibrous bands. There is deposition of lipid crystals forming rosettes within the fat lobules. The inflammation is very scanty, and the lesion is rapidly progressive. Mostly the lesion is limited to subcutaneous fat but rarely can involve visceral fat, which leads to increased mortality.[71],[72],[73],[74],[75],[76]

Pancreatic panniculitis

Clinically, pancreatic panniculitis overlaps quite closely with that of nodular panniculitis (Weber–Christian type). Patients with nodular panniculitis have also been described with lipasemia or amylasemia but with no evidence of pancreatic disease.

The earliest histological change of pancreatic panniculitis is fat necrosis, which is probably initiated by circulating enzymes (lipases) and varying degrees of calcification. These changes may be focal, emphasizing the need for a larger surgical biopsy and serial sectioning. Inflammatory infiltrate is relatively sparse around the areas of fat necrosis, consisting mainly of neutrophils. Areas with fat necrosis show “ghost-like” adipocytes having fine basophilic intracytoplasmic granular calcification. Later on, however, the infiltrate becomes granulomatous.[77],[78],[79],[80],[81] It should also be borne in mind that cutaneous aspergillosis and mucormycosis can closely mimic pancreatic panniculitis on histopathology, owing to the presence of “ghost-like” adipocytes.[8]

 Others



Eosinophilic panniculitis

Eosinophilic panniculitis is characterized by a prominent infiltration of subcutaneous fat with eosinophils. It can be seen in patients with drug dependence, psychiatric illness, atopy, asthma, malignancies, immune complex vasculitis, and sarcoidosis. Eosinophilic panniculitis in EN is perhaps its most confusing presentation.[82],[83],[84],[85]

 Lobular Panniculitis With Vasculitis



Erythema nodosum leprosum

This is a Type 2 reaction occurs most commonly in lepromatous leprosy (LL) followed by borderline leprosy. Clinically, the patients present with tender erythematous plaques or nodules [Figure 4]b. It is often accompanied with fever, malaise, and arthralgia.

Histologically, the lesions show neutrophilic infiltrates superimposed on chronic multibacillary leprosy. An associated necrotizing vasculitis affecting arterioles, venules, and capillaries can be seen in some cases of erythema nodosum leprosum (ENL) [Figure 11]. Sometimes, the neutrophilic infiltration is scanty with a predominance of macrophages containing fragmented bacilli.[86],[87],[88],[89] In a series of 32 patients with ENL, 22 patients developed ENL before the commencement of chemotherapy, thereby highlighting the fact that development of ENL in the absence of chemotherapy is not an infrequent event and early recognition of this event is important. The authors concluded that ENL should be looked upon as a manifestation of leprosy, and not essentially as a mere complication of its therapy.[87]{Figure 11}

Lucio's reaction

The Lucio's reaction is vascular manifestation of diffuse LL, in which no treatment or inadequate treatment is given. In contrast to ENL, endothelial proliferation and thrombosis in the medium-sized vessels of the dermis and subcutis are seen leading to luminal obliteration. Strong acid-fast bacteria positivity is found in the walls and endothelium of normal looking vessels and also in vessels showing proliferative changes.[90],[91],[92]

Neutrophilic lobular panniculitis associated with rheumatoid arthritis

Rheumatoid arthritis may be accompanied by cutaneous manifestations such as rheumatoid granuloma, leukocytoclastic vasculitis, and rheumatoid neutrophilic dermatitis.

Histopathology shows a predominantly lobular panniculitis, severe necrosis of the adipocytes in the lobule, and an inflammatory infiltrate composed of neutrophils, foamy histiocytes, and multinucleate giant cells. Small cystic spaces lined by amorphous eosinophilic material were seen in some cases as an early expression of lipomembranous or membranocystic panniculitis. Neutrophilic lobular panniculitis should be differentiated from the suppurative variant of EN, in which numerous neutrophils extend into the lobule from fibrous septa.[93],[94]

Erythema induratum of Bazin/nodular vasculitis

NV is a multifactorial syndrome with lobular panniculitis and vasculitis, in which tuberculosis may or may not be one of its many etiologies. Thus, erythema induratum/NV complex is classified into two variants: EIB type and NV or erythema induratum of Whitfield type [Figure 1]c. The Bazin type has a tuberculous etiology.

Histologically, EIB/NV shows predominantly acute suppurative or granulomatous panniculitis with fibrin deposition in small vessel walls along with karyorrhexis and hemorrhage. In a series of 101 skin biopsies from 86 patients with clinicopathologic diagnosis of EIB, it was seen that vasculitis was obvious in 90.1% cases. Nearly 46.5% biopsies revealed mostly lobular panniculitis with necrotizing vasculitis of the centrilobular small vessels, 12.8% biopsies exhibited mostly lobular panniculitis with vasculitis involving both large septal veins and small centrilobular venules, 11.8% biopsies showed lobular panniculitis with vasculitis involving mainly large septal veins, without any involvement of other lobular or septal vessels, 9.9% biopsies showed predominantly lobular panniculitis with vasculitis involving large septal vessels, both arteries and veins, and necrotizing vasculitis of the centrilobular venules, and 8.9% biopsies displayed lobular panniculitis with vasculitis involving larger septal vessels (both arteries and veins), but without any involvement of the smaller centrilobular vessels. There were 9.9% biopsies with a predominantly lobular panniculitis but without any obvious evidence of septal or lobular vasculitis, even in serial sections.[28] The differential diagnosis of EIB/NV typically includes PAN, which can be differentiated clinically and by the presence of all stages of the lesion in the biopsy.[95],[96],[97]

Subcutaneous panniculitis-like T-cell lymphoma

SPTCL is a very rare form of skin lymphoma and accounts for <1% of all non-Hodgkin's lymphomas. It is localized primarily to the subcutaneous adipose tissue without the involvement of the lymph nodes.

SPTCL is histologically characterized by neoplastic lymphoid cells infiltrating mainly the lobules of the subcutaneous tissue with or without the involvement of the epidermis and dermis. The neoplastic lymphoid cells have hyperchromatic, angulated nuclei, scant cytoplasm, and indistinct cell borders. In addition, interspersed histiocytes, plasma cells, neutrophils, mitoses, apoptotic bodies, karyorrhectic debris, and areas of fat necrosis are also seen. The atypical cells often show rimming of individual fat cells [Figure 12].{Figure 12}

Based on T-cell receptor (TCR) phenotype and immunophenotypic characteristics, there are two types of SPTCL. One is TCR-αβ, associated with an indolent course and the neoplastic T-cells are usually CD4−, CD8+, and CD56−. The second type is TCR-γδ, characterized by rapid clinical deterioration and CD4−, CD8−, and CD56+ neoplastic T-cells. Nowadays, diagnosis of SPTCL is used for patients with TCR-αβ, while TCR-γδ type is designated as cutaneous gamma/delta positive T-cell lymphoma (Cγδ-TCL).[98],[99],[100],[101],[102],[103],[104]

 Conclusion



Some important points to remember in cases with clinically suspected panniculitis:

A deep elliptical surgical biopsy is essential to have adequate subcutaneous fat A detailed clinical history is indispensable for histopathological assessment Step-wise serials to identify the pattern of panniculitis, i.e., septal, lobular, mixed, with or without vasculitis are often mandatory A careful search for the diagnostic clues should be made, for example, crystals in lipocytes or histiocytes Special stains in “obscure” suppurative or granulomatous panniculitis cases, to see fungal hyphae or spores, are obligatory Sometimes, polarizing the sections is important to look for sclerosing lipogranulomas or factitial panniculitis Advising follow-up biopsies of representative lesions are imperative to study stages of evolution of the diseases, especially in cases with vasculitis.

In any given case of panniculitis, a diagnostic algorithm should be followed to reach to an appropriate histopathologic diagnosis with adequate clinical information [Table 2] and [Table 3].{Table 2}{Table 3}

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

References

1Peters MS, Su WP. Panniculitis. Dermatol Clin 1992;10:37-57.
2Diaz Cascajo C, Borghi S, Weyers W. Panniculitis: Definition of terms and diagnostic strategy. Am J Dermatopathol 2000;22:530-49.
3Ter Poorten MC, Thiers BH. Panniculitis. Dermatol Clin 2002;20:421-33.
4Patterson JW. Differential diagnosis of panniculitis. Adv Dermatol 1991;6:309-29.
5Henry F, Piérard GE. Current classification of panniculitis. Rev Med Liege 1998;53:603-9.
6Segura S, Requena L. Anatomy and histology of normal subcutaneous fat, necrosis of adipocytes, and classification of the panniculitides. Dermatol Clin 2008;26:419-24.
7McNutt NS, Moreno A, Contreas F. Inflammatory diseases of subcutaneous fat. In: Elder DE, editor. Lever's Histopathology of Skin. 10th ed. Philadelphia: Lippincott; Williams and Wilkins; 2009. p. 509-36.
8Requena L, Yus ES. Panniculitis. Part I. Mostly septal panniculitis. J Am Acad Dermatol 2001;45:163-83.
9Requena L. Normal subcutaneous fat, necrosis of adipocytes and classification of the panniculitides. Semin Cutan Med Surg 2007;26:66-70.
10Eberhard BA, Ilowite NT. Panniculitis and lipodystrophy. Curr Opin Rheumatol 2002;14:566-70.
11Ahmed B, Estey E, Manning J, David C, Keating MJ, Kantarjian H, et al. Anaplastic large cell lymphoma with involvement of the pancreas presenting as panniculitis in a patient with a history of acute myeloid leukemia – Case report and review of the literature. Haematologica 2006;91 12 Suppl: ECR55.
12Chao YY, Yang LJ. Dermatomyositis presenting as panniculitis. Int J Dermatol 2000;39:141-4.
13Sabroe RA, Wallington TB, Kennedy CT. Dermatomyositis treated with high-dose intravenous immunoglobulins and associated with panniculitis. Clin Exp Dermatol 1995;20:164-7.
14Rosenberg AS, Morgan MB. Disseminated acanthamoebiasis presenting as lobular panniculitis with necrotizing vasculitis in a patient with AIDS. J Cutan Pathol 2001;28:307-13.
15Bourré-Tessier J, Fortin C, Belisle A, Desmarais E, Choquette D, Senécal JL. Disseminated Histoplasma capsulatum infection presenting with panniculitis and focal myositis in rheumatoid arthritis treated with etanercept. Scand J Rheumatol 2009;38:311-6.
16Ege G, Akman H, Cakiroglu G. Mesenteric panniculitis associated with abdominal tuberculous lymphadenitis: A case report and review of the literature. Br J Radiol 2002;75:378-80.
17Blanc F, Robert C, Fadlallah JP, Moulonguet I, Maurin F, Civatte J. Necrotic ulcer patch mimicking factitious panniculitis disclosing systemic lupus erythematosus. Ann Dermatol Venereol 1990;117:836-8.
18Zundler S, Erber R, Agaimy A, Hartmann A, Kiesewetter F, Strobel D, et al. Pancreatic panniculitis in a patient with pancreatic-type acinar cell carcinoma of the liver-case report and review of literature. BMC Cancer 2016;16:130.
19Bogart MM, Milliken MC, Patterson JW, Padgett JK. Pancreatic panniculitis associated with acinic cell adenocarcinoma: A case report and review of the literature. Cutis 2007;80:289-94.
20Preiss JC, Faiss S, Loddenkemper C, Zeitz M, Duchmann R. Pancreatic panniculitis in an 88-year-old man with neuroendocrine carcinoma. Digestion 2002;66:193-6.
21Requena L, Yus ES. Erythema nodosum. Dermatol Clin 2008;26:425-38.
22Gilchrist H, Patterson JW. Erythema nodosum and erythema induratum (nodular vasculitis): Diagnosis and management. Dermatol Ther 2010;23:320-7.
23Schwartz RA, Nervi SJ. Erythema nodosum: A sign of systemic disease. Am Fam Physician 2007;75:695-700.
24Pileckyte M, Griniute R. Erythema nodosum association with malignant lymphoma. Medicina (Kaunas) 2003;39:438-42.
25Rosai J, editor. Skin. In: Surgical Pathology. 9th ed. St. Louis: Mosby; 2009. p. 93-130.
26Weedon D, editor. Panniculits. In: Skin Pathology. 3rd ed. London. Churchill Livingstone; 2010. p. 460-77.
27Mascaró JM Jr., Baselga E. Erythema induratum of Bazin. Dermatol Clin 2008;26:439-45.
28Segura S, Pujol RM, Trindade F, Requena L. Vasculitis in erythema induratum of Bazin: A histopathologic study of 101 biopsy specimens from 86 patients. J Am Acad Dermatol 2008;59:839-51.
29Laguna C, Alegre V, Pérez A. Superficial migratory thrombophlebitis: A clinical and histologic review of 8 cases. Actas Dermosifiliogr 2008;99:390-5.
30Chanet V, Amarger S, Pons B, Déchelotte P, Ruivard M, Philippe P. Nodular thrombophlebitis and granulomatous systemic disease. Rev Med Interne 2007;28:416-9.
31Sanz Vico MD, De Diego V, Sánchez Yus E. Erythema nodosum versus nodular vasculitis. Int J Dermatol 1993;32:108-12.
32Marker M, Ortiz-Urda S, Lilgenau N, Rappersberger K. Erythema induratum-nodular vasculitis. J Dtsch Dermatol Ges 2004;2:206-14.
33Boonchai W, Suthipinittharm P, Mahaisavariya P. Panniculitis in tuberculosis: A clinicopathologic study of nodular panniculitis associated with tuberculosis. Int J Dermatol 1998;37:361-3.
34Heinemann C, Kaatz M, Elsner P. Erythema induratum of Bazin and Poncet's disease – Successful treatment with antitubercular drugs. J Eur Acad Dermatol Venereol 2003;17:334-6.
35Peyrí J, Moreno A, Marcoval J. Necrobiosis lipoidica. Semin Cutan Med Surg 2007;26:87-9.
36Lowitt MH, Dover JS. Necrobiosis lipoidica. J Am Acad Dermatol 1991;25:735-48.
37Balbir-Gurman A, Braun-Moscovici Y. Scleroderma – New aspects in pathogenesis and treatment. Best Pract Res Clin Rheumatol 2012;26:13-24.
38Requena L, Fernández-Figueras MT. Subcutaneous granuloma annulare. Semin Cutan Med Surg 2007;26:96-9.
39Muhlbauer JE. Granuloma annulare. J Am Acad Dermatol 1980;3:217-30.
40Palmer DG. The anatomy of the rheumatoid lesion. Br Med Bull 1995;51:286-95.
41García-Patos V. Rheumatoid nodule. Semin Cutan Med Surg 2007;26:100-7.
42Ziff M. The rheumatoid nodule. Arthritis Rheum 1990;33:761-7.
43Bettoni L, Bani L, Airò P. Rheumatoid nodules: The importance of a correct differential diagnosis. Eur Ann Allergy Clin Immunol 2011;43:95-6.
44Morgan AJ, Schwartz RA. Cutaneous polyarteritis nodosa: A comprehensive review. Int J Dermatol 2010;49:750-6.
45Subbanna PK, Singh NV, Swaminathan RP. Cutaneous polyarteritis nodosa: A rare isolated cutaneous vasculitis. Indian Dermatol Online J 2012;3:21-4.
46Ishiguro N, Kawashima M. Cutaneous polyarteritis nodosa: A report of 16 cases with clinical and histopathological analysis and a review of the published work. J Dermatol 2010;37:85-93.
47Furukawa F. Cutaneous polyarteritis nodosa: An update. Ann Vasc Dis 2012;5:282-8.
48Nakamura T, Kanazawa N, Ikeda T, Yamamoto Y, Nakabayashi K, Ozaki S, et al. Cutaneous polyarteritis nodosa: Revisiting its definition and diagnostic criteria. Arch Dermatol Res 2009;301:117-21.
49Luis Rodríguez-Peralto J, Carrillo R, Rosales B, Rodríguez-Gil Y. Superficial thrombophlebitis. Semin Cutan Med Surg 2007;26:71-6.
50Samlaska CP, James WD. Superficial thrombophlebitis. I. Primary hypercoagulable states. J Am Acad Dermatol 1990;22:975-89.
51Samlaska CP, James WD. Superficial thrombophlebitis. II. Secondary hypercoagulable states. J Am Acad Dermatol 1990;23:1-18.
52Leu HJ. Thrombosis and phlebitis of superficial veins. Differential histopathologic diagnosis. Pathologe 1995;16:386-90.
53Fraga J, García-Díez A. Lupus erythematosus panniculitis. Dermatol Clin 2008;26:453-63.
54Izumi AK. Lupus erythematosus panniculitis. Clin Dermatol 1985;3:69-78.
55Peters MS, Su WP. Lupus erythematosus panniculitis. Med Clin North Am 1989;73:1113-26.
56Guissa VR, Trudes G, Jesus AA, Aikawa NE, Romiti R, Silva CA. Lupus erythematosus panniculitis in children and adolescents. Acta Reumatol Port 2012;37:82-5.
57Weingartner JS, Zedek DC, Burkhart CN, Morrell DS. Lupus erythematosus panniculitis in children: Report of three cases and review of previously reported cases. Pediatr Dermatol 2012;29:169-76.
58Rao TN, Ahmed K, Venkatachalam K. Lupus erythematosus profundus. Indian J Dermatol Venereol Leprol 2010;76:448.
59Requena C, Sanmartín O, Requena L. Sclerosing panniculitis. Dermatol Clin 2008;26:501-4.
60Kirsner RS, Pardes JB, Eaglstein WH, Falanga V. The clinical spectrum of lipodermatosclerosis. J Am Acad Dermatol 1993;28:623-7.
61Requena L, Ferrándiz C. Sclerosing postirradiation panniculitis. Dermatol Clin 2008;26:505-8.
62Moreno A, Marcoval J, Peyri J. Traumatic panniculitis. Dermatol Clin 2008;26:481-3.
63Shellagi N, Rodrigues G. Traumatic panniculitis of the right thigh: A case report. Oman Med J 2011;26:436-7.
64Nagore E, Sánchez-Motilla JM, Rodríguez-Serna M, Vilata JJ, Aliaga A. Lipoatrophia semicircularis – A traumatic panniculitis: Report of seven cases and review of the literature. J Am Acad Dermatol 1998;39:879-81.
65Winkelmann RK, Barker SM. Factitial traumatic panniculitis. J Am Acad Dermatol 1985;13:988-94.
66Mitra S, Dove J, Somisetty SK. Subcutaneous fat necrosis in newborn-an unusual case and review of literature. Eur J Pediatr 2011;170:1107-10.
67Savic DM, Stojanovic ND, Stankovic VD, Stojkovic AK, Canovic DS, Ninkovic SM, et al. Subcutaneous fat necrosis in newborns. Med Glas (Zenica) 2012;9:429-31.
68Singh A, Sharma S, Vermani S, Khunger N. Subcutaneous fat necrosis of the new born. Indian J Pathol Microbiol 2007;50:849-50.
69Kenani N, Mebaza A, Denguezli M, Sriha B, Belajouza C, Nouira R. Subcutaneous fat necrosis of the newborn. Eur J Dermatol 2007;17:99-100.
70Singalavanija S, Limponsanurak W, Wannaprasert T. Subcutaneous fat necrosis of the newborn. J Med Assoc Thai 2007;90:1214-20.
71Reddy CR, Murthy DP, Reddy GE. Sclerema neonatorum. Indian Pediatr 1970;7:244-6.
72Fretzin DF, Arias AM. Sclerema neonatorum and subcutaneous fat necrosis of the newborn. Pediatr Dermatol 1987;4:112-22.
73Milunsky A, Levin SE. Sclerema neonatorum: A clinical study of 79 cases. S Afr Med J 1966;40:638-41.
74Zeb A, Darmstadt GL. Sclerema neonatorum: A review of nomenclature, clinical presentation, histological features, differential diagnoses and management. J Perinatol 2008;28:453-60.
75Gupta A, Sur AM, Junnarkar RV, Nardas U. Sclerema neonatorum: Clinical and autopsy report. Indian Pediatr 1968;5:192-201.
76Dasgupta A, Ghosh RN, Pal RK, Mukherjee N. Sclerema neonatorum – Histopathologic study. Indian J Pathol Microbiol 1993;36:45-7.
77García-Romero D, Vanaclocha F. Pancreatic panniculitis. Dermatol Clin 2008;26:465-70.
78Poelman SM, Nguyen K. Pancreatic panniculitis associated with acinar cell pancreatic carcinoma. J Cutan Med Surg 2008;12:38-42.
79Fernández-Jorge B, Verea-Hernando MM, Alvarez-Rodríguez R, Paradela S, Fonseca E. Pancreatic panniculitis. Report of a case and review of literature. An Med Interna 2006;23:431-4.
80Narváez J, Bianchi MM, Santo P, de la Fuente D, Ríos-Rodriguez V, Bolao F, et al. Pancreatitis, panniculitis, and polyarthritis. Semin Arthritis Rheum 2010;39:417-23.
81Braun-Falco O, Hohenleutner U, von der Helm D, Landthaler M. Pancreatogenic panniculitis 2 case reports with a literature review. Hautarzt 1989;40:778-81.
82Liu Y, Xiao SX, Wang JM, Peng ZH, Feng YG, Lei XB, et al. Eosinophilic panniculitis: Report of three cases. Int J Dermatol 2006;45:1412-4.
83Masferrer E, Martin-Ezquerra G, Martínez-Escala E, Pujol RM, Giménez-Arnau A. Eosinophilic panniculitis triggered by intramuscular penicillin and occupational setting. Allergy 2011;66:436-7.
84Koca Kalkan I, Baccioglu A, Kalpaklioglu F. Eosinophilic panniculitis: A new form of local reaction with specific immunotherapy. Allergy 2009;64:1692-3.
85Adame J, Cohen PR. Eosinophilic panniculitis: Diagnostic considerations and evaluation. J Am Acad Dermatol 1996;34:229-34.
86Meyerson MS. Erythema nodosum leprosum. Int J Dermatol 1996;35:389-92.
87Rea TH, Levan NE. Erythema nodosum leprosum in a general hospital. Arch Dermatol 1975;111:1575-80.
88Cuevas J, Rodríguez-Peralto JL, Carrillo R, Contreras F. Erythema nodosum leprosum: Reactional leprosy. Semin Cutan Med Surg 2007;26:126-30.
89Kahawita IP, Lockwood DN. Towards understanding the pathology of erythema nodosum leprosum. Trans R Soc Trop Med Hyg 2008;102:329-37.
90Kaur C, Thami GP, Mohan H. Lucio phenomenon and Lucio leprosy. Clin Exp Dermatol 2005;30:525-7.
91Saúl A, Novales J. Lucio-Latapí leprosy and the Lucio phenomenon. Acta Leprol 1983;1:115-32.
92Sehgal VN. Lucio's phenomenon/erythema necroticans. Int J Dermatol 2005;44:602-5.
93Tran TA, DuPree M, Carlson JA. Neutrophilic lobular (pustular) panniculitis associated with rheumatoid arthritis: A case report and review of the literature. Am J Dermatopathol 1999;21:247-52.
94Sutra-Loubet C, Carlotti A, Guillemette J, Wallach D. Neutrophilic panniculitis. J Am Acad Dermatol 2004;50:280-5.
95Cho KH, Lee DY, Kim CW. Erythema induratum of Bazin. Int J Dermatol 1996;35:802-8.
96Sharon V, Goodarzi H, Chambers CJ, Fung MA, Armstrong AW. Erythema induratum of Bazin. Dermatol Online J 2010;16:1.
97Wiebels D, Turnbull K, Steinkraus V, Böer A. Erythema induratum Bazin. “Tuberculid” or tuberculosis? Hautarzt 2007;58:237-40.
98Parveen Z, Thompson K. Subcutaneous panniculitis-like T-cell lymphoma: Redefinition of diagnostic criteria in the recent World Health Organization-European Organization for Research and Treatment of Cancer classification for cutaneous lymphomas. Arch Pathol Lab Med 2009;133:303-8.
99Rolinski M, Brown VL, Ratnavel R. Subcutaneous panniculitis-like T-cell lymphoma. J Eur Acad Dermatol Venereol 2010;24:241-2.
100Francis A, Criton S, Acharya S, Shojan A, Philip RM. Subcutaneous panniculitis-like T-cell lymphoma. Indian J Dermatol 2010;55:290-2.
101Singh A, Kumar J, Kapur S, Ramesh V. Subcutaneous panniculitis-like T-cell cutaneous lymphoma. Indian J Dermatol Venereol Leprol 2008;74:151-3.
102Gallardo F, Pujol RM. Subcutaneous panniculitis-like T-cell lymphoma and other primary cutaneous lymphomas with prominent subcutaneous tissue involvement. Dermatol Clin 2008;26:529-40.
103Wang CY, Su WP, Kurtin PJ. Subcutaneous panniculitic T-cell lymphoma. Int J Dermatol 1996;35:1-8.
104Yung A, Snow J, Jarrett P. Subcutaneous panniculitic T-cell lymphoma and cytophagic histiocytic panniculitis. Australas J Dermatol 2001;42:183-7.